Journal: Journal of Inflammation Research

Article Title: Anthrahydroquinone-2,6-Disulfonate Restores Lung Function in COPD Through Keap1/Nrf2 Pathway Activation

doi: 10.2147/JIR.S524429

Figure Lengend Snippet: Effects of AH 2 QDS on the LPS+CSE-induced HBE cell migration, apoptosis and inflammatory factor expression. ( A and B ) Transwell and quantitative plots of each experimental group. (20×, scale bar=200μm). ( C ) Levels of apoptosis in the experimental groups. ( D – G ) Levels of IL-6, IL-33, IL-1β, and TNF-α. n≥3, ns: not statistically significant, * P ˂0.05, ** P ˂0.01, *** P ˂0.001, **** P ˂0.0001.

Article Snippet: Apoptosis rates in each experimental group of cells were determined using an Annexin V-FITC/PI Apoptosis Detection Kit (cat#40302, Yeasen, Shanghai, China), a widely recognized method for distinguishing apoptotic cells based on phosphatidylserine externalization and membrane integrity.

Techniques: Migration, Expressing

Journal: Bioactive Materials

Article Title: ROS-responsive & scavenging NO nanomedicine for vascular diseases treatment by inhibiting endoplasmic reticulum stress and improving NO bioavailability

doi: 10.1016/j.bioactmat.2024.03.010

Figure Lengend Snippet: Schematic illustration of ROS-responsive & scavenging nanomedicines for vascular injury diseases treatment. With excellent ROS-responsive & scavenging ability, nanomedicines can achieve exogenous delivery of NO while inhibiting the conversion of NO to ONOO − caused by elevated ROS, thereby improving the bioavailability of NO and providing a basis for the differential regulation of endothelial cells (ECs) and smooth muscle cells (SMCs). In addition, this nanomedicine will regulate the homeostatic imbalance of Ca 2+ induced by ER stress and inhibit subsequent mitochondrial dysfunction and apoptosis.

Article Snippet: Fetal bovine serum was purchased from Zhejiang Baidi Biotechnology Co., Ltd. Fluo-4 AM (40704 ES), Rhod-2 AM (40776 ES), Mitotracker (40742 ES) and Annexin V-FITC/PI apoptosis kit (40302 ES) were purchased from Yeasen Biotechnology Co., Ltd (Shanghai, China).

Techniques:

Journal: Bioactive Materials

Article Title: ROS-responsive & scavenging NO nanomedicine for vascular diseases treatment by inhibiting endoplasmic reticulum stress and improving NO bioavailability

doi: 10.1016/j.bioactmat.2024.03.010

Figure Lengend Snippet: Regulation of ER stress by NPs in HUVECs. (A) Gene ontology (GO) classification and (B) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs between H 2 O 2 and PBA&NO NP group. (C) Possible mechanism of PBA&NO NP. (D) Cytoplasmic calcium level detected by Fluo-4 AM probe, (E) mitochondrial calcium level detected by Rhod-2 AM probe and Mitotracker co-staining, and (F) mitochondrial membrane potential detected by JC-10 assay. The nuclei were labeled by Hoechst 33258, scale bars, 100 μm. (G) Flow cytometry analysis of HUVECs after different treatments for 24 h using annexin V-FITC and PI assay. The inner image was the percentage of apoptotic cells. (H) WB of ER stress-related protein (XBP-1) and apoptosis-related proteins (BAX, Bcl2 and c-cas3) after different treatments. The concentrations of NO and PBA in the NPs were both 10 μM. Data are presented as mean ± SD (n = 3). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Fetal bovine serum was purchased from Zhejiang Baidi Biotechnology Co., Ltd. Fluo-4 AM (40704 ES), Rhod-2 AM (40776 ES), Mitotracker (40742 ES) and Annexin V-FITC/PI apoptosis kit (40302 ES) were purchased from Yeasen Biotechnology Co., Ltd (Shanghai, China).

Techniques: Staining, Membrane, Labeling, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: Umbilical cord blood-derived neutrophils possess higher viability than peripheral blood derived neutrophils

doi: 10.62347/HQIP2227

Figure Lengend Snippet: Cytokine cocktail activation enhanced the ovarian cancer cell killing activity of UCBN. A. The viability of ovarian cancer cells line OVCAR3 co-cultured with activated UCBN (donors of UCB-7, UCB-8, UCB-9) or PBN (PB-6, PB-7, PB-8) assessed by CCK-8 assay. *P < 0.05, **P < 0.01. B. The viability of ovarian cancer cells line OVCAR3 treated with conditioned medium (CM) of activated UCBN (donors of UCB-2, UCB-3, UCB-6, UCB-7, UCB-8) or PBN (donors of PB-1, PB-2, PB-3, PB-6, PB-7) assessed by CCK-8 assay. *P < 0.05, **P < 0.01. C. Represent images of immunofluorescence staining of EdU of ovarian cancer cells line OVCAR3 treated with conditioned medium (CM) of activated UCBN or PBN. Bar = 50 μm. D. The proliferation capacity of ovarian cancer cells line OVCAR3 treated with conditioned medium (CM) of activated UCBN or PBN assessed by EdU anaylsis. E. The apoptosis of ovarian cancer cells line OVCAR3 treated with conditioned medium (CM) from activated UCBN or PBN by Annexin-V PI analysis. N = 3 donors each group, **P < 0.01, ***P < 0.001.

Article Snippet: Cells were suspended in 100 ul Binding Buffer and stained with Annexin V-FITC/PI apoptosis detection kit (Cat No. 40302, YEASEN) following the manufacturer’s protocol.

Techniques: Activation Assay, Activity Assay, Cell Culture, CCK-8 Assay, Immunofluorescence, Staining